Tnf^tm2Gkl
Targeted Allele Detail

Summary

• Symbol: Tnf^tm2Gkl
• Name: tumor necrosis factor; targeted mutation 2, George Kollias
• MGI ID: MGI:2388715
• Synonyms: TNF^deltaARE
• Gene: Tnf Location: Chr17:35418357-35420983 bp, - strand Genetic Position: Chr17, 18.59 cM
• Alliance: Tnf^tm2Gkl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54056
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 69 bp encompassing the AU-rich elements (ARE) were deleted by homologous recombination of a floxed neo cassette and subsequent in vitro cre mediated excision. The resultant allele contained a single loxP site in place of the ARE sequence. ELISA of homozygous mutant mice showed protein levels higher than in wild-type mice, indicating a suppressive role of the AREs. (J:54056)

Disease models

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnf Mutation: 48 strains or lines available

References

• Original: J:54056 Kontoyiannis D, et al., Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98
• All: 48 reference(s)