Afp^tm1Ibmm
Targeted Allele Detail

Summary

• Symbol: Afp^tm1Ibmm
• Name: alpha fetoprotein; targeted mutation 1, Claude Szpirer
• MGI ID: MGI:2388722
• Gene: Afp Location: Chr5:90638596-90656766 bp, + strand Genetic Position: Chr5, 44.77 cM
• Alliance: Afp^tm1Ibmm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79500
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of exon 1 and all of exons 2 and 3 were replaced by a cassette containing both lacZ and neo. The insertion was designed to delete the 3' promoter (in intron 1) and leave lacZ under the control of the 5' promoter located upstream of exon 1. Beta-galactosidase expression was verfied by staining. The absence of endogenous transcript and protein was confirmed by Northern and Western blot analyses. (J:79500)

Expression

In Mice Carrying this Mutation:	24 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Afp Mutation: 32 strains or lines available

References

• Original: J:79500 Gabant P, et al., Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility. Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12865-70
• All: 6 reference(s)