Cdcs8^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Cdcs8^C57BL/6J
• Name: cytokine deficiency colitis susceptibility 8; C57BL/6J
• MGI ID: MGI:2388739
• QTL: Cdcs8 Location: Chr12:81689083-81689229 bp Genetic Position: Chr12, cM position of peak correlated region/allele: 37.86 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers colitis susceptibility compared to C3H/HeJBir. (J:78917)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

This allele is consistent with a recessive or additive mode of inheritance.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:78917

A genome scan using 69 microsatellite markers at an average resolution of 20.4 cM was performed on 2 backcross populations to identify QTLs associated with colitis susceptibility. The backcross populations used are (C3H/HeJBir-Il10^tm1Cgn x C57BL/6J-Il10^tm1Cgn)F1 x C3H/HeJBir-Il10^tm1Cgn and (C57BL/6J-Il10^tm1Cgn x C3H/HeJBir-^Il10tm1Cgn)F1 x C57BL/6J-Il10^tm1Cgn. They are referred to by the authors as N2(C3H)-Il10^-/- and N2(B6)-Il10^-/-, respectively. Parental strain C3H/HeJBir-Il10^tm1Cgn exhibits severe lesions of the cecum and colon as early as 4 weeks of age whereas parental strain C57BL/6J-Il10^tm1Cgn exhibits mild lesions. Colitis susceptibility loci mapped to mouse Chromosome 12 (Cdcs7 and Cdcs8) in the N2(C3H)-Il10^-/- backcross, and to mouse Chromosomes 4 (Cdcs9) and 5 (Cdcs10) in the N2(B6)-Il10^-/- backcross. Cdcs7 is an epistatic locus with peak colitis linkage at 38 cM on chromosome 12 at D12Mit214. The QTL range spans 10 cM - 50 cM. Homozygosity for C3H/HeJBir-derived allelesat Cdcs7 in conjunction with C3H/HeJBir-derived alleles at D3Mit257 contribute to colitis susceptibility. Cdcs8 maps to the same location as Cdcs7 (38 cM on chromosome 12) with a LOD score of 3.2 but exhibits a different mode of inheritance than Cdcs7.C57BL/6J-derived alleles confer colitis susceptibility in a recessive or additive fashion at Cdcs8. On mouse Chromosome 4, Cdcs9 maps to 71 cM at D4Mit13 with a QTL range of 60 cM - 80 cM. C3H/HejBir-derived alleles confer colitis susceptibility in a dominant or additive fashion at Cdcs9. On mouse Chromosome 5, Cdcs10 maps to 45 cM at D5Mit205 with a QTL range of 36 cM - 60 cM. C3H/HejBir-derived alleles confer colitis susceptibility in a dominant or additive fashion at Cdcs10. The following suggestive loci were also identified: Chromosome 3 at D3Mit257 (C3H/HeJBir-derived allele conferring recessive or additive susceptibility), Chromosome 8 at D8Mit200 (C57BL/6J-derived allele conferring dominant or additive susceptibility), and Chromosome 13 at D13Mit179 (C57BL/6J-derived allele conferring susceptibility).

References

• Original: J:78917 Mahler M, et al., Genetics of Colitis Susceptibility in IL-10-Deficient Mice: Backcross versus F2 Results Contrasted by Principal Component Analysis. Genomics. 2002 Sep;80(3):274
• All: 1 reference(s)