Summary |
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Variant origin |
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Variant description |
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Phenotypes |
View phenotypes and curated references for all genotypes (concatenated display).
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Expression |
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Notes |
Candidate Genes
Microarray analysis was used to identify candidate genes for colitis susceptibility QTLs. Messenger RNA was analyzed for expression differences between colitis susceptible inbred strain C3H/HeJBir-Il10tm1Cgn and colitis resistant inbred strain C57BL/6J-Il10tm1Cgn. Mapping and Phenotype information for this QTL, its variants and associated markersJ:78917A genome scan using 69 microsatellite markers at an average resolution of 20.4 cM was performed on 2 backcross populations to identify QTLs associated with colitis susceptibility. The backcross populations used are (C3H/HeJBir-Il10tm1Cgn x C57BL/6J-Il10tm1Cgn)F1 x C3H/HeJBir-Il10tm1Cgn and (C57BL/6J-Il10tm1Cgn x C3H/HeJBir-Il10tm1Cgn)F1 x C57BL/6J-Il10tm1Cgn. They are referred to by the authors as N2(C3H)-Il10-/- and N2(B6)-Il10-/-, respectively. Parental strain C3H/HeJBir-Il10tm1Cgn exhibits severe lesions of the cecum and colon as early as 4 weeks of age whereas parental strain C57BL/6J-Il10tm1Cgn exhibits mild lesions. Colitis susceptibility loci mapped to mouse Chromosome 12 (Cdcs7 and Cdcs8) in the N2(C3H)-Il10-/- backcross, and to mouse Chromosomes 4 (Cdcs9) and 5 (Cdcs10) in the N2(B6)-Il10-/- backcross. Cdcs7 is an epistatic locus with peak colitis linkage at 38 cM on chromosome 12 at D12Mit214. The QTL range spans 10 cM - 50 cM. Homozygosity for C3H/HeJBir-derived allelesat Cdcs7 in conjunction with C3H/HeJBir-derived alleles at D3Mit257 contribute to colitis susceptibility. Cdcs8 maps to the same location as Cdcs7 (38 cM on chromosome 12) with a LOD score of 3.2 but exhibits a different mode of inheritance than Cdcs7.C57BL/6J-derived alleles confer colitis susceptibility in a recessive or additive fashion at Cdcs8. On mouse Chromosome 4, Cdcs9 maps to 71 cM at D4Mit13 with a QTL range of 60 cM - 80 cM. C3H/HejBir-derived alleles confer colitis susceptibility in a dominant or additive fashion at Cdcs9. On mouse Chromosome 5, Cdcs10 maps to 45 cM at D5Mit205 with a QTL range of 36 cM - 60 cM. C3H/HejBir-derived alleles confer colitis susceptibility in a dominant or additive fashion at Cdcs10. The following suggestive lociwere also identified: chromosome 3 at D3Mit257 (C3H/HeJBir-derived allele conferring recessive or additive susceptibility), chromosome 8 at D8Mit200 (C57BL/6J-derived allele conferring dominant or additive susceptibility), and chromosome 13 at D13Mit179 (C57BL/6J-derived allele conferring susceptibility). |
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References |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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