Art2b^tm1Fkn
Targeted Allele Detail

Summary

• Symbol: Art2b^tm1Fkn
• Name: ADP-ribosyltransferase 2b; targeted mutation 1, Friedrich Koch-Nolte
• MGI ID: MGI:2388827
• Synonyms: ART2.2-
• Gene: Art2b Location: Chr7:101226177-101234807 bp, - strand Genetic Position: Chr7, 54.61 cM
• Alliance: Art2b^tm1Fkn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79607
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The coding region was disrupted by a hygromycin selection cassette inserted by homologous recombination. This construct was used to transfect mice in which the Art2a^tm1Fkn allele had already been created Transcript was undetected in homozygous mutant mice by RT-PCR analysis of T cells isolated from lymph nodes. FACS analysis of thymocytes and lymphocytes indicated a lack of protein expression on the cell surface. (J:79607)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Art2b Mutation: 23 strains or lines available

Notes

The targeting vector was electroporated into J1 ES cells carrying Art2a^tm1Fkn.

References

• Original: J:79607 Ohlrogge W, et al., Generation and characterization of ecto-ADP-ribosyltransferase ART2.1/ART2.2-deficient mice. Mol Cell Biol. 2002 Nov;22(21):7535-42
• All: 13 reference(s)