Canx^tm1.1Den
Targeted Allele Detail

Summary

• Symbol: Canx^tm1.1Den
• Name: calnexin; targeted mutation 1.1, Angela Denzel
• MGI ID: MGI:2388910
• Synonyms: sCnx
• Gene: Canx Location: Chr11:50184788-50216500 bp, - strand Genetic Position: Chr11, 30.46 cM
• Alliance: Canx^tm1.1Den page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79604
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The floxed selection cassette that replaced a fragment encompassing 137 bp of exon 4, intron 4, and all of exon 5 was excised from Canx^tm1Den by in vivo cre mediated recombination in the germline. Expression analysis identified a truncated protein, lacking residues encoded by exons 4, 5, and 6. The deleted region included cysteine residues 161 and 195 which form a disulfide bond, and tyrosine 165 and lysine 167 which are both involved with the glucose binding pocket. The deletion reportedly did not directly affect the ER targeting region, allowing for the possiblity for the truncated protein to localize to the ER. (J:79604)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Canx Mutation: 55 strains or lines available

References

• Original: J:79604 Denzel A, et al., Early postnatal death and motor disorders in mice congenitally deficient in calnexin expression. Mol Cell Biol. 2002 Nov;22(21):7398-404
• All: 2 reference(s)