Zap70^tm2.1Mmal
Targeted Allele Detail

Summary

• Symbol: Zap70^tm2.1Mmal
• Name: zeta-chain (TCR) associated protein kinase; targeted mutation 2.1, Marie Malissen
• MGI ID: MGI:2388918
• Synonyms: ZAP-70 Y315F
• Gene: Zap70 Location: Chr1:36800912-36821899 bp, + strand Genetic Position: Chr1, 15.41 cM, cytoband A4-C1
• Alliance: Zap70^tm2.1Mmal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71099
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: An intronic floxed neo cassette was removed by crossing to a cre-expressing deleter strain, leaving behind a tyrosine to phenylalanine knock-in mutation at position 315 (Y315F). Expression of the mutant allele was confirmed by sequence analysis of RT-PCR products from thymus of mutant animals. (J:71099)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zap70 Mutation: 55 strains or lines available

References

• Original: J:71099 Magnan A, et al., T cell development and T cell responses in mice with mutations affecting tyrosines 292 or 315 of the ZAP-70 protein tyrosine kinase. J Exp Med. 2001 Aug 20;194(4):491-505
• All: 1 reference(s)