Csf3r^{tm4(Epor)Link}
Targeted Allele Detail

Summary

• Symbol: Csf3r^{tm4(Epor)Link}
• Name: colony stimulating factor 3 receptor; targeted mutation 4, Daniel C Link
• MGI ID: MGI:2388978
• Synonyms: GE, G:EpoR
• Gene: Csf3r Location: Chr4:125918343-125938233 bp, + strand Genetic Position: Chr4, 59.77 cM
• Alliance: Csf3r^{tm4(Epor)Link} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79825
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 16 and 17 of the endogenous gene, which encode the cytoplasmic domain, were replaced by an Epor cDNA fragment. The resultant allele encodes a chimeric receptor with the endogenous extracytoplasmic (ligand binding) and transmembrane regions adjoined to the Epor cytoplasmic (signaling) region. Flow cytometric and ligand binding assays indicated that the chimeric receptor had normal binding affinity and was expressed on the surface of homozygous mutant neutrophils at a level approximately 80% of normal expression. (J:79825)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Csf3r Mutation: 42 strains or lines available

References

• Original: J:79825 Semerad CL, et al., A role for G-CSF receptor signaling in the regulation of hematopoietic cell function but not lineage commitment or differentiation. Immunity. 1999 Aug;11(2):153-61
• All: 4 reference(s)