Sirpa^tm1Nog
Targeted Allele Detail

Summary

• Symbol: Sirpa^tm1Nog
• Name: signal-regulatory protein alpha; targeted mutation 1, Tetsuya Noguchi
• MGI ID: MGI:2388979
• Synonyms: SHPS-1-cyto(-), SHPS-1 MT, SirpaCT^-, SIRPalpha^-, SIRPalpha MT, SIRP CT^-
• Gene: Sirpa Location: Chr2:129434755-129474148 bp, + strand Genetic Position: Chr2, 63.19 cM, cytoband F3
• Alliance: Sirpa^tm1Nog page

Presence of megakaryocytes in the spleen of 11 week old Sirpa^tm1Nog/Sirpa^tm1Nog mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79824
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 7 and 8 were replaced by a neomycin selection cassette inserted by homologous recombination. The deleted region encoded the majority of the cytoplasmic region. A low level of production of a truncated protein was identified by expression analyses of brain tissue and peritoneal macrophages. Biochemical assays showed that the mutant protein did not undergo tyrosine phosphorylation nor did it associate with Hcph. (J:79592, J:79824)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Sirpa Mutation: 51 strains or lines available

References

• Original: J:79824 Inagaki K, et al., SHPS-1 regulates integrin-mediated cytoskeletal reorganization and cell motility. EMBO J. 2000 Dec 15;19(24):6721-31
• All: 44 reference(s)