Tg(Tagln-cre/ERT2)1Feil
Transgene Detail

Summary

• Symbol: Tg(Tagln-cre/ERT2)1Feil
• Name: transgene insertion 1, Robert Feil
• MGI ID: MGI:2389039
• Synonyms: SM-CreER^T2(tg), Tg(Tagln-cre/ESR1)1Feil
• Transgene: Tg(Tagln-cre/ERT2)1Feil Location: unknown
• Alliance: Tg(Tagln-cre/ERT2)1Feil page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:69325
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Tagln-cre/ERT2)1Feil expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Tagln

• Mutation details: An allele encoding a tissue-specific and ligand-dependent recombinase was generated by adjoining cre recombinase/modified estrogen receptor (ESR1) fusion sequence to 5.5 kb of the transgelin promoter and regulatory region. The ESR1 sequence contained engineered missense mutations at positions 400, 543, 540 which substituted the ability to bind the endogenous ligand with the ability to bind tamoxifen (J:79835). The Tagln promoter directed expression in smooth muscle cells. Transgenic expression was detected in smooth muscle tissue but was characterized as being low and mosaic. Recombination was tamoxifen-dependent. (J:69325)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tagln (species not specified) Summary of all recombinase alleles driven by Tagln. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:69325 Kuhbandner S, et al., Temporally controlled somatic mutagenesis in smooth muscle. Genesis. 2000 Sep;28(1):15-22
• All: 12 reference(s)