Tagln^{tm1(cre/ERT2)Feil}
Targeted Allele Detail

Summary

• Symbol: Tagln^{tm1(cre/ERT2)Feil}
• Name: transgelin; targeted mutation 1, Robert Feil
• MGI ID: MGI:2389040
• Synonyms: SM22alphaCew-ER^T2, SM-CreERT2(ki), SM-Cre ER^T2(ki), SM-CreER^T2(ki), Tagln^{tm1(cre/ESR1)Feil}
• Gene: Tagln Location: Chr9:45840926-45847356 bp, - strand Genetic Position: Chr9, 25.36 cM
• Alliance: Tagln^{tm1(cre/ERT2)Feil} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69325
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tagln^{tm1(cre/ERT2)Feil} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tagln (MGI:106012)

• Mutation details: An allele encoding a tissue specific and ligand dependent recombinase was generated by inserting cre recombinase/modified estrogen receptor (ESR1) fusion sequence into the endogenous allele. The ESR1 sequence contained engineered missense mutations at positions 400, 543, 540 which substituted the ability to bind the endogenous ligand with the ability to bind tamoxifen (J:79835). Histochemical analysis of mutant mice treated with tamoxifen showed expression of cre in smooth muscle tissue. (J:69325)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tagln (mouse) Summary of all recombinase alleles driven by Tagln. Comparative matrix view of recombinase activities Alliance mouse Tagln gene page

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tagln Mutation: 28 strains or lines available

References

• Original: J:69325 Kuhbandner S, et al., Temporally controlled somatic mutagenesis in smooth muscle. Genesis. 2000 Sep;28(1):15-22
• All: 36 reference(s)