Blnk^tm1Achn
Targeted Allele Detail

Summary

• Symbol: Blnk^tm1Achn
• Name: B cell linker; targeted mutation 1, Andrew C Chan
• MGI ID: MGI:2389137
• Synonyms: BLNK-
• Gene: Blnk Location: Chr19:40917371-40982664 bp, - strand Genetic Position: Chr19, 34.26 cM
• Alliance: Blnk^tm1Achn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58805
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 1 was disrupted by homologous recombination of a construct containing GFP, a floxed neo gene, and a mutated start codon (ATC). While the GFP coding region was linked to its own Kozak sequence and start codon, fluorescence was undetected in splenocytes and bone marrow cells derived from heterozygous mutant mice. Immunoblot analysis on homozygote bone marrow cells failed to detect Blnk protein. (J:58805)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Blnk Mutation: 78 strains or lines available

References

• Original: J:58805 Pappu R, et al., Requirement for B cell linker protein (BLNK) in B cell development. Science. 1999 Dec 3;286(5446):1949-54
• All: 10 reference(s)