Camk2a^tm4Sva
Targeted Allele Detail

Summary

• Symbol: Camk2a^tm4Sva
• Name: calcium/calmodulin-dependent protein kinase II alpha; targeted mutation 4, Alcino Silva
• MGI ID: MGI:2389254
• Synonyms: TT305/6VA
• Gene: Camk2a Location: Chr18:61058704-61121224 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Camk2a^tm4Sva page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79714
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The endogenous sequence encoding threonine residues at positions 305 and 306 was modified to encode valine and alanine by homologous recombination of a site directed mutagenized construct. The replacement of the threonine residues with nonphosphorylatable amino acids was designed to inhibit autophosphorylation of the targeted residues. Western blot analysis of homozygous mutant mice showed that normal sized protein was present at a level approximately two fold lower than that observed in wild-type mice. Further analysis using antibodies directed against phosphorylated threonine residues 305 and 286 showed immunoreactivity for only the latter. A single loxP site was left within the locus. (J:79714)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Camk2a Mutation: 135 strains or lines available

References

• Original: J:79714 Elgersma Y, et al., Inhibitory Autophosphorylation of CaMKII Controls PSD Association, Plasticity, and Learning. Neuron. 2002 Oct 24;36(3):493-505
• All: 5 reference(s)