Modm^BALB/cByJ
QTL Variant Detail

Summary

• QTL variant: Modm^BALB/cByJ
• Name: modifier of methylation; BALB/cByJ
• MGI ID: MGI:2389304
• QTL: Modm Location: unknown Genetic Position: Chr13, Syntenic

Variant origin

• Strain of Specimen: BALB/cByJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased DNA methylation compared to C57BL/6ByJ. (J:67850)

Notes

Animals homozygous for BALB/cByJ-derived alleles at Modm exhibited 80%-60% methylation at the transgene locus.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:67850

QTL mapping was performed on a cross between (BALB/cJ x C57BL/6J)F1 females and E36 transgenic C57BL/6J males. The hepatitis B transgene is differentially methylated depending upon parent of origin. Paternal transmission to a C57BL/6J background results in undermethylation while paternal transmission to a BALB/cJ background results in hypermethylation. Maternal transmission to any genetic background results in methylation. 102 microsatellite markers at an average spacing of 11 cM was typed in 88 (BALB/cJx C57BL/6J)F1 x E36 progeny for QTL mapping. A major locus, Modm (modifier of methylation), was detected on mouse Chromosome 13 spanning 30 cM - 45 cM with a maximum LOD = 16.5 near D13Mit21 and D13Mit97. This locus includes the site of the transgene insertion. Modm was confirmed in 4 CXB (C = BALB/cByJ, B = C57BL/6ByJ) recombinant inbred (RI) strains. CXB RI strains homozygous for BALB/cByJ-derived alleles at Modm exhibited 80%-60% methylation at the transgene locus whereas CXB RI strains homozygous for C57BL/6ByJ-derived alleles exhibit 30%-20% methylation at the transgene locus.

References

• Original: J:67850 Valenza-Schaerly P, et al., A dominant modifier of transgene methylation is mapped by qtl analysis to mouse chromosome 13. Genome Res. 2001 Mar;11(3):382-8
• All: 1 reference(s)