Ak1^tm1Bew
Targeted Allele Detail

Summary

• Symbol: Ak1^tm1Bew
• Name: adenylate kinase 1; targeted mutation 1, Be Wieringa
• MGI ID: MGI:2389509
• Synonyms: AK1^-
• Gene: Ak1 Location: Chr2:32511770-32525070 bp, + strand Genetic Position: Chr2, 22.09 cM
• Alliance: Ak1^tm1Bew page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66101
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 through 5, encoding the ATP binding site, were replaced with a hygromycin selection cassette inserted by homologous recombination. Transcript was undetected by Northern blot analysis of skeletal muscle, heart, and brain tissue obtained from homozygous mutant mice. Zymogram analysis of various tissues confirmed the absence of functional protein. (J:66101)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ak1 Mutation: 32 strains or lines available

References

• Original: J:66101 Janssen E, et al., Adenylate kinase 1 gene deletion disrupts muscle energetic economy despite metabolic rearrangement. EMBO J. 2000 Dec 1;19(23):6371-81
• All: 14 reference(s)