Tg(CMV-IgkvaD11)ACat
Transgene Detail

Summary

• Symbol: Tg(CMV-IgkvaD11)ACat
• Name: transgene insertion A, Antonio Cattaneo
• MGI ID: MGI:2429312
• Synonyms: AD10 A, AD11-VK, CMV-VK alphaD11, Tg(CMV-Igk@aD11)ACat, Tg(Igk)1Cat, VK-alphaD11
• Transgene: Tg(CMV-IgkvaD11)ACat Location: unknown
• Alliance: Tg(CMV-IgkvaD11)ACat page

Dilation of lateral ventricles and cholinergic deficit in aged Tg(CMV-IghvaD11)DCat/0 Tg(CMV-IgkvaD11)ACat/0 mice

Show the 4 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CMV-IgkvaD11)ACat expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  rat	Igkv	"The gene encodes a recombined variable region from the rat anti-nerve growth factor monoclonal antibody, alphaD11."

• Mutation details: The transgenic construct included a recombined Igk variable region encoding the light chain of the rat anti-nerve growth factor monoclonal antibody, alphaD11. The human immunoglobulin kappa constant region (IGKC) was included downstream to enable the detection of the transgenic antibodies against murine immunoglobulins. The early region promoter (-601 to -16) of human CMV reportedly drove expression of the transgene in non-neuronal tissures as well as in neuronal tissues including the cortex, hippocampus, thalamus, spinal cord, retina, olfactory bulb, and cerebellum. Line A produces lower expression of the light chain transgene than line B. (J:79946)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Founder lines A and B were generated. Expression was reported to be higher in line A than line B.

Double transgenic mice expressing both light and heavy chains of the alphaD11 antibody were generated by breeding mice harboring this transgene with mice carrying Tg(CMV-IghvaD11)CCat.

References

• Original: J:79946 Ruberti F, et al., Phenotypic knockout of nerve growth factor in adult transgenic mice reveals severe deficits in basal forebrain cholinergic neurons, cell death in the spleen, and skeletal muscle dystrophy. J Neurosci. 2000 Apr 1;20(7):2589-601
• All: 14 reference(s)