Slc9a1^tm1Smb
Targeted Allele Detail

Summary

• Symbol: Slc9a1^tm1Smb
• Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1; targeted mutation 1, Sheila M Bell
• MGI ID: MGI:2429323
• Synonyms: Nhe1 -, Nhe1^-
• Gene: Slc9a1 Location: Chr4:133097022-133151013 bp, + strand Genetic Position: Chr4, 66.25 cM, cytoband D3-E
• Alliance: Slc9a1^tm1Smb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54705
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of exon 2 was replaced by a neomycin selection cassette inserted by homologous recombination. The deleted region encoded transmembrane domains 6 and 7 (amino acids 217 through 275). Two aberrant transcripts were detected in mutant mice by Northern blot and RT-PCR analyses. Analysis indicated that a 613 bp transcript resulted from readthrough of the neo cassette and a 380 bp transcript underwent splicing of codon 198 to exon 3. Biochemical analysis, based on pH recover after an acute acid load, of acinar cells indicated a functional ablation of the endogenous protein. (J:54705)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc9a1 Mutation: 41 strains or lines available

References

• Original: J:54705 Bell SM, et al., Targeted disruption of the murine Nhe1 locus induces ataxia, growth retardation, and seizures. Am J Physiol. 1999 Apr;276(4 Pt 1):C788-95
• All: 21 reference(s)