Gclc^tm1Dwn
Targeted Allele Detail

Summary

• Symbol: Gclc^tm1Dwn
• Name: glutamate-cysteine ligase, catalytic subunit; targeted mutation 1, Daniel W Nebert
• MGI ID: MGI:2429419
• Synonyms: Gclc(-)
• Gene: Gclc Location: Chr9:77661817-77701767 bp, + strand Genetic Position: Chr9, 43.36 cM, cytoband D-E
• Alliance: Gclc^tm1Dwn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66499
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An Hprt mini gene replaced a portion of exon 4 and all of exons 5 and 6. While translation of the targeted allele may be possible, in which case a truncated protein lacking all but 161 of the 637 amino acids would be produced, Western blot analysis showed protein levels to be decreased by approximately 50% in heterozygous mice. An assay of endogenous enzyme activity correspondingly showed an approximate 50% reduction in hepatic tissue obtained from heterozygotes. (J:66499)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gclc Mutation: 20 strains or lines available

References

• Original: J:66499 Dalton TP, et al., Knockout of the mouse glutamate cysteine ligase catalytic subunit (Gclc) gene: embryonic lethal when homozygous, and proposed model for moderate glutathione deficiency when heterozygous. Biochem Biophys Res Commun. 2000 Dec 20;279(2):324-9
• All: 1 reference(s)