Hsf2^tm1Ijb
Targeted Allele Detail

Summary

• Symbol: Hsf2^tm1Ijb
• Name: heat shock factor 2; targeted mutation 1, Ivor J Benjamin
• MGI ID: MGI:2429646
• Synonyms: Hsf2-
• Gene: Hsf2 Location: Chr10:57362481-57389231 bp, + strand Genetic Position: Chr10, 29.19 cM, cytoband B3-B4
• Alliance: Hsf2^tm1Ijb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79910
• Parent Cell Line: KG-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 200 bp exon containing the translational start codon and a 5' portion of the DNA binding domain was replaced with a neomycin selection cassette. Transcript was undetected by Northern blot analysis of brain, heart, kidney, liver, and lung tissue. Western blot analysis of testis extract indicated a lack of protein in homozygous mutant mice. To confirm that this absence was due to a disrupted allele and not the labile nature of the protein, MEFs treated with proteasome inhibitors were analyzed and similar results were obtained. (J:79910)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsf2 Mutation: 38 strains or lines available

References

• Original: J:79910 McMillan DR, et al., Heat shock transcription factor 2 is not essential for embryonic development, fertility, or adult cognitive and psychomotor function in mice. Mol Cell Biol. 2002 Nov;22(22):8005-14
• All: 2 reference(s)