Cabin1^tm1.1Che
Targeted Allele Detail

Summary

• Symbol: Cabin1^tm1.1Che
• Name: calcineurin binding protein 1; targeted mutation 1.1, Jianzhu Chen
• MGI ID: MGI:2429675
• Synonyms: Cabin1DeltaC
• Gene: Cabin1 Location: Chr10:75481946-75600175 bp, - strand Genetic Position: Chr10, 38.56 cM
• Alliance: Cabin1^tm1.1Che page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72760
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The floxed neomycin selection cassette that had replaced the last two exons of the endogenous gene was excised from Cabin1^tm1Che via in vivo cre mediated recombination. The deleted region encoded the carboxy terminal which includes the calcineurin, calmodulin, and MEF2 binding domains. Northern blot analysis of homozygous mutant mice identified a transcript of the anticipated size in brain and thymus tissue. While RT-PCR and subsequent sequence analysis showed that translation of this transcript could result in a truncated protein that contained 4 new amino acid residues in place of the endogenous carboxy terminal, the lack of an amino terminal antibody prevented direct confirmation of such a peptide. (J:72760)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cabin1 Mutation: 114 strains or lines available

References

• Original: J:72760 Esau C, et al., Deletion of Calcineurin and Myocyte Enhancer Factor 2 (MEF2) Binding Domain of Cabin1 Results in Enhanced Cytokine Gene Expression in T Cells. J Exp Med. 2001 Nov 19;194(10):1449-59
• All: 1 reference(s)