Raf1^tm1Zim
Targeted Allele Detail

Summary

• Symbol: Raf1^tm1Zim
• Name: v-raf-leukemia viral oncogene 1; targeted mutation 1, Andreas Zimmer
• MGI ID: MGI:2429683
• Synonyms: Craf-1^neo2
• Gene: Raf1 Location: Chr6:115595530-115653596 bp, - strand Genetic Position: Chr6, 53.62 cM, cytoband C3
• Alliance: Raf1^tm1Zim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50696
• Parent Cell Line: MPI-II (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A hypomorphic allele was generated by disrupting the first coding exon (exon 2) with a neo cassette. While Western blot analysis showed an absence of normal protein (74 kDA), low levels of a 62 kDa protein were identified. Sequence analysis of mRNA showed excision of the targeted exon and identified potential start codons in exon 3. Biochemical assays showed no kinase activity in nonstimulated mutant fibroblasts and a 90% reduction in activity in stimulated fibroblasts relative to wild-type samples. (J:50696)

Expression

In Structures Affected by this Mutation:

15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Raf1 Mutation: 117 strains or lines available

References

• Original: J:50696 Wojnowski L, et al., Craf-1 protein kinase is essential for mouse development. Mech Dev. 1998 Aug;76(1-2):141-9
• All: 3 reference(s)