Mef2a^tm1Eno
Targeted Allele Detail

Summary

• Symbol: Mef2a^tm1Eno
• Name: myocyte enhancer factor 2A; targeted mutation 1, Eric N Olson
• MGI ID: MGI:2429769
• Synonyms: Mef2a^-
• Gene: Mef2a Location: Chr7:66880911-67022606 bp, - strand Genetic Position: Chr7, 36.72 cM
• Alliance: Mef2a^tm1Eno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79973
• Parent Cell Line: KG-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The second coding exon was replaced with a neomycin selection cassette. RT-PCR confirmed the absence of normal transcript. While no normal protein was detected, a truncated protein was identified by Western blot analysis of homozygous mutant heart tissue. The mutant protein was translated from an aberrantly spliced mRNA in which exon 1 joined sequence downstream of neo. Gel mobility shift assays indicated an of ablation of endogenous DNA binding activity in homozygous mutant mice. (J:79973)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mef2a Mutation: 36 strains or lines available

References

• Original: J:79973 Naya FJ, et al., Mitochondrial deficiency and cardiac sudden death in mice lacking the MEF2A transcription factor. Nat Med. 2002 Nov;8(11):1303-9
• All: 8 reference(s)