Gk^tm1Wjc
Targeted Allele Detail

Summary

• Symbol: Gk^tm1Wjc
• Name: glycerol kinase; targeted mutation 1, William J Craigen
• MGI ID: MGI:2429928
• Gene: Gk Location: ChrX:84745543-84820425 bp, - strand Genetic Position: ChrX, 39.32 cM, cytoband C-D
• Alliance: Gk^tm1Wjc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43449
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encompassing all of exons 13 and 14 as well as a portion of exon 15 was replaced with a neomycin selection cassette. mRNA was undetected by Northern blot analysis of liver tissue obtained from homozygous mutant mice. A biochemical assay showed glycerol kinase activity to be reduced by over 95% in liver, kidney, and brown fat. (J:43449)

Disease models

Expression

In Mice Carrying this Mutation:	4 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gk Mutation: 12 strains or lines available

References

• Original: J:43449 Huq AH, et al., X-linked glycerol kinase deficiency in the mouse leads to growth retardation, altered fat metabolism, autonomous glucocorticoid secretion and neonatal death. Hum Mol Genet. 1997 Oct;6(11):1803-9
• All: 7 reference(s)