Grk3^tm1Rjl
Targeted Allele Detail

Summary

• Symbol: Grk3^tm1Rjl
• Name: G protein-coupled receptor kinase 3; targeted mutation 1, Robert J Lefkowitz
• MGI ID: MGI:2429934
• Synonyms: GRK3 (-)
• Gene: Grk3 Location: Chr5:113058344-113163518 bp, - strand Genetic Position: Chr5, 55.29 cM
• Alliance: Grk3^tm1Rjl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43423
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 7 and 8 (encoding part of the protein kinase catalytic domain) were replaced with a PGK-neomycin resistance cassette (in the antisense orientation) by homologous recombination. Included in the deleted region was sequence encoding the ATP binding motif of catalytic subdomain I. While transcript was detected by Northern blot analysis of homozygous mutant mice, the absence of protein was verified by Western blot analysis. (J:43423)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grk3 Mutation: 34 strains or lines available

References

• Original: J:43423 Peppel K, et al., G protein-coupled receptor kinase 3 (GRK3) gene disruption leads to loss of odorant receptor desensitization. J Biol Chem. 1997 Oct 10;272(41):25425-8
• All: 14 reference(s)