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Tcrgtm1Iku
Targeted Allele Detail
Summary
Symbol: Tcrgtm1Iku
Name: T cell receptor gamma chain; targeted mutation 1, Koichi Ikuta
MGI ID: MGI:2429947
Synonyms: TCR-Vgamma4/6 -, TCR Vgamma4/6 KO, Vgammadelta
Gene: Tcrg  Location: Chr13:19362212-19540646 bp, + strand  Genetic Position: Chr13, cytoband A2-A3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:80156
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsA targeting vector containing a Tcrg-V2 gene modified to lack a restriction site, a floxed Tcrg-V4 gene, and a Tcrg-V3 gene also modified to lack a restriction site was electroporated into ES cells. The ES cells were then transfected with cre recombinase, resulting in the excision of Tcrg-V4. Tcrg-V2 was left adjacent to Tcrg-V3 (with single loxP site in between). Southern blot analysis indicated that only a portion of the targeting vector recombined as the modified Tcrg-V2 and the endogenous Tcrg-V3 (not the modified Tcrg-V3) were present. (J:39791)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tcrg Mutation:  1 strain or line available
References
Original:  J:80156 Sunaga S, et al., Efficient removal of loxP-flanked DNA sequences in a gene-targeted locus by transient expression of Cre recombinase in fertilized eggs. Mol Reprod Dev. 1997 Feb;46(2):109-13
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory