Acr^tm1Wen
Targeted Allele Detail

Summary

• Symbol: Acr^tm1Wen
• Name: acrosin prepropeptide; targeted mutation 1, Wolfgang Engel
• MGI ID: MGI:2429958
• Synonyms: Acr^-
• Gene: Acr Location: Chr15:89452549-89458790 bp, + strand Genetic Position: Chr15, 44.96 cM, cytoband E-F
• Alliance: Acr^tm1Wen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:38658
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 2 was disrupted by the insertion of a neomycin selection cassette. Aberrant transcripts were detected by Northern blot and RT-PCR analyses of mutant mice. The use of various cDNA fragments and sequence analysis indicated that the mutant transcripts lacked portions of exon 2 and contained neomycin sequence. Southern blot analysis confirmed that the aberrant splicing was not a result of incorrect recombination. No protein was detected by immunostaining of sperm obtained from homozygous mutant mice. (J:38658)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Acr Mutation: 49 strains or lines available

References

• Original: J:38658 Adham IM, et al., Spermatozoa lacking acrosin protein show delayed fertilization. Mol Reprod Dev. 1997 Mar;46(3):370-6
• All: 4 reference(s)