Ccnb2^tm1Thnt
Targeted Allele Detail

Summary

• Symbol: Ccnb2^tm1Thnt
• Name: cyclin B2; targeted mutation 1, Tim Hunt
• MGI ID: MGI:2429974
• Gene: Ccnb2 Location: Chr9:70314974-70328829 bp, - strand Genetic Position: Chr9, 39.53 cM, cytoband D
• Alliance: Ccnb2^tm1Thnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47430
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 4 and 5 were replaced with a neomycin selection cassette. The deleted sequence encoded the conserved cyclin box involved in the binding of cell cycle division 2. While neither mRNA nor protein was detected in homozygous mutant mice by Northern and Western blot analyses, 5' and amino terminal fragments were expected in vivo. (J:47430)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccnb2 Mutation: 31 strains or lines available

References

• Original: J:47430 Brandeis M, et al., Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4344-9
• All: 4 reference(s)