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Gata1tm5(GATA3)Sho
Targeted Allele Detail
Summary
Symbol: Gata1tm5(GATA3)Sho
Name: GATA binding protein 1; targeted mutation 5, Stuart Orkin
MGI ID: MGI:2429992
Synonyms: G1G3ki
Gene: Gata1  Location: ChrX:7825504-7842844 bp, - strand  Genetic Position: ChrX, 3.59 cM, cytoband A2
Alliance: Gata1tm5(GATA3)Sho page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:47295
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsSequence extending from the start codon in exon 2 to the EcoRI site in exon 3 was replaced with a construct containing human GATA3 cDNA sequence followed by a floxed PGK-neo cassette. Northern blot analyses of cell lines derived from mutant embryos indicated that a transcript comprised of both GATA3 and Gata1 sequence was expressed in erythroid cells (but not in myeloid cells). The presence of a protein translated from GATA3 sequence was verified by immunostaining. No Gata1 protein was detected. (J:47295)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gata1 Mutation:  45 strains or lines available
References
Original:  J:47295 Tsai FY, et al., Knock-in mutation of transcription factor GATA-3 into the GATA-1 locus: partial rescue of GATA-1 loss of function in erythroid cells. Dev Biol. 1998 Apr 15;196(2):218-27
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory