Ccr5^tm1Brv
Targeted Allele Detail

Summary

• Symbol: Ccr5^tm1Brv
• Name: C-C motif chemokine receptor 5; targeted mutation 1, Rodrigo Bravo
• MGI ID: MGI:2429994
• Synonyms: ccr5^-
• Gene: Ccr5 Location: Chr9:123921557-123934153 bp, + strand Genetic Position: Chr9, 75.05 cM, cytoband F
• Alliance: Ccr5^tm1Brv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46941
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette into exon 2 via homologous recombination replaced sequence encoding all 7 transmembrane domains. Whereas mRNA transcribed from other chemokine (C-C motif) receptor loci was shown to be present at normal levels, endogenous transcript was undetected in homozygous mutant mice by Northern blot analysis. The expression of other CC-chemokine receptor genes, Ccr1, Ccr2, Ccr3, and Ccr4, was not affected. (J:46941)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccr5 Mutation: 31 strains or lines available

Notes

ES cell line = CJ7 (129S1/Sv-p⁺ Tyr⁺ Kitl⁺) or R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl⁺).

References

• Original: J:46941 Zhou Y, et al., Impaired macrophage function and enhanced T cell-dependent immune response in mice lacking CCR5, the mouse homologue of the major HIV-1 coreceptor. J Immunol. 1998 Apr 15;160(8):4018-25
• All: 4 reference(s)