Slc4a1^tm1Ahc
Targeted Allele Detail

Summary

• Symbol: Slc4a1^tm1Ahc
• Name: solute carrier family 4 (anion exchanger), member 1; targeted mutation 1, Athar H Chishti
• MGI ID: MGI:2441657
• Synonyms: band 3^-/-
• Gene: Slc4a1 Location: Chr11:102239646-102256107 bp, - strand Genetic Position: Chr11, 66.29 cM, cytoband D
• Alliance: Slc4a1^tm1Ahc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35637
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 3. The insertion disrupted the coding region 12 codons downstream of the erythroid-specific start codon. While no protein was detected in red blood cells obtained from homozygous mutant mice, translation of the kidney specific isoform, which is initiated from a start codon downstream of the inserted neo cassette, was verified by Western blot analysis of kidney lysates. (J:35637)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc4a1 Mutation: 54 strains or lines available

References

• Original: J:35637 Southgate CD, et al., Targeted disruption of the murine erythroid band 3 gene results in spherocytosis and severe haemolytic anaemia despite a normal membrane skeleton. Nat Genet. 1996 Oct;14(2):227-30
• All: 4 reference(s)