Gja5^tm1Kwi
Targeted Allele Detail

Summary

• Symbol: Gja5^tm1Kwi
• Name: gap junction protein, alpha 5; targeted mutation 1, Klaus Willecke
• MGI ID: MGI:2445238
• Synonyms: Cx40-
• Gene: Gja5 Location: Chr3:96939718-96960950 bp, + strand Genetic Position: Chr3, 42.04 cM
• Alliance: Gja5^tm1Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46429
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 5' region of exon 2, which is the only coding exon, was replaced with a neomycin selection cassette. No mRNA was detected by Northern blot analysis of heart or lung tissue obtained from homozygous mutant mice and the absence of protein was verified by Western blot analysis of liver tissue. (J:46429, J:83400)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gja5 Mutation: 19 strains or lines available

References

• Original: J:46429 Kirchhoff S, et al., Reduced cardiac conduction velocity and predisposition to arrhythmias in connexin40-deficient mice. Curr Biol. 1998 Feb 26;8(5):299-302
• All: 33 reference(s)