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Plcb3tm1Sko
Targeted Allele Detail
Summary
Symbol: Plcb3tm1Sko
Name: phospholipase C, beta 3; targeted mutation 1, Britt Skogseid
MGI ID: MGI:2445310
Gene: Plcb3  Location: Chr19:6931081-6951738 bp, - strand  Genetic Position: Chr19, 5.1 cM, cytoband B
Alliance: Plcb3tm1Sko page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:51860
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA 4 kb genomic fragment encompassing exons 11 through 17 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region (encoding amino acids 338 through 681) included portions of the X and Y domains, which are putatively essential for enzymatic activity, and a nuclear targeting signal. Western blot analysis showed protein levels to be reduced by approximately 50% in lung tissue obtained from heterozygous mutant mice. (J:51860)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 4 assay results
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Plcb3 Mutation:  50 strains or lines available
References
Original:  J:51860 Wang S, et al., Targeted disruption of the mouse phospholipase C beta3 gene results in early embryonic lethality. FEBS Lett. 1998 Dec 18;441(2):261-5
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory