Plcb3tm1Sko
Targeted Allele Detail
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Symbol: |
Plcb3tm1Sko |
Name: |
phospholipase C, beta 3; targeted mutation 1, Britt Skogseid |
MGI ID: |
MGI:2445310 |
Gene: |
Plcb3 Location: Chr19:6931081-6951738 bp, - strand Genetic Position: Chr19, 5.1 cM, cytoband B
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Alliance: |
Plcb3tm1Sko page
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Germline Transmission: |
Earliest citation of germline transmission:
J:51860
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Parent Cell Line: |
E14.1 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A 4 kb genomic fragment encompassing exons 11 through 17 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region (encoding amino acids 338 through 681) included portions of the X and Y domains, which are putatively essential for enzymatic activity, and a nuclear targeting signal. Western blot analysis showed protein levels to be reduced by approximately 50% in lung tissue obtained from heterozygous mutant mice.
(J:51860)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Plcb3 Mutation: |
50 strains or lines available
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Original: |
J:51860 Wang S, et al., Targeted disruption of the mouse phospholipase C beta3 gene results in early embryonic lethality. FEBS Lett. 1998 Dec 18;441(2):261-5 |
All: |
1 reference(s) |
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