Selp^tm2Hyn
Targeted Allele Detail

Summary

• Symbol: Selp^tm2Hyn
• Name: selectin, platelet; targeted mutation 2, Richard O Hynes
• MGI ID: MGI:2445316
• Synonyms: DeltaCT
• Gene: Selp Location: Chr1:163942833-163977595 bp, + strand Genetic Position: Chr1, 71.42 cM
• Alliance: Selp^tm2Hyn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51068
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The endogenous locus was disrupted by homologous recombination of a construct containing two engineered stop codons followed by a human growth hormone (hGH) polyadenylation signal and neo. The stop codons were inserted after the first 9 nt encoding the cytoplasmic domain. In spite of the detection of both truncated and elongated transcripts by Northern blot analysis, Western blot analysis showed an absence of cytomplasmic domain peptide sequence in platelet and lung tissue lysates obtained from homozygous mutant mice. (J:51068)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Selp Mutation: 57 strains or lines available

References

• Original: J:51068 Hartwell DW, et al., Role of P-selectin cytoplasmic domain in granular targeting in vivo and in early inflammatory responses. J Cell Biol. 1998 Nov 16;143(4):1129-41
• All: 5 reference(s)