Rapgef1^{Gt(pGT1.8geo)5Pgr}
Gene trapped Allele Detail

Summary

• Symbol: Rapgef1^{Gt(pGT1.8geo)5Pgr}
• Name: Rap guanine nucleotide exchange factor (GEF) 1; gene trap 5, Peter Gruss
• MGI ID: MGI:2445403
• Synonyms: C3G^gt
• Gene: Rapgef1 Location: Chr2:29509732-29630376 bp, + strand Genetic Position: Chr2, 20.32 cM, cytoband B
• Alliance: Rapgef1^{Gt(pGT1.8geo)5Pgr} page

Mutation origin

• Mutant Cell Line: F82
• Germline Transmission: Earliest citation of germline transmission: J:80219
• Parent Cell Line: MPI-II (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector Type: gene trap
• Mutation details: The gene trap insertion is located in the 59 kb intron 1, approximately 16 kb 3' to exon 1. The predicted protein deduced from this fusion gene contains the first 19 amino acids of the endogenous protein fused to beta-galactosidase and neomycin phosphotransferase. This fusion protein will lack the carboxy-terminal CDC25-type catalytic domain, the 4 central Crk SH3-binding domains and the central p130Cas SH3-binding domain. However, RT-PCR and western blot analysis has shown that this allele generates less than 1% mRNA containing all coding exons and no more than 5% normal protein suggesting that some transcripts splice around the gene trap insertion site. Therefore, this is a hypomorphic rather than a null allele. (J:80219)

Expression

In Mice Carrying this Mutation:	50 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rapgef1 Mutation: 192 strains or lines available

References

• Original: J:47982 Voss AK, et al., Efficiency assessment of the gene trap approach. Dev Dyn. 1998 Jun;212(2):171-80
• All: 5 reference(s)