Rr320^tm2Mkg
Targeted Allele Detail

Summary

• Symbol: Rr320^tm2Mkg
• Name: regulatory region 320; targeted mutation 2, Mark Groudine
• MGI ID: MGI:2445411
• Synonyms: deltaHS2+neo, Hbb-ar^tm2Mkg
• Gene: Rr320 Location: Chr7:103501973-103526752 bp, + strand Genetic Position: Chr7, Syntenic
• Alliance: Rr320^tm2Mkg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:28981
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A 1.1 kb genomic region containing the 5' DNase I hypersensitive site 2 (HS2) was replaced with an FRT site flanked neomycin resistance gene cassette via homologous recombination. Successful gene targeting was confirmed by Southern blot analysis of mutant animals. (J:28981)

Disease models

Expression

In Mice Carrying this Mutation:	36 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr320 Mutation: 0 strains or lines available

References

• Original: J:28981 Fiering S, et al., Targeted deletion of 5'HS2 of the murine beta-globin LCR reveals that it is not essential for proper regulation of the beta-globin locus. Genes Dev. 1995 Sep 15;9(18):2203-13
• All: 1 reference(s)