Rb1^tm1Jyjw
Targeted Allele Detail

Summary

• Symbol: Rb1^tm1Jyjw
• Name: RB transcriptional corepressor 1; targeted mutation 1, Jean YJ Wang
• MGI ID: MGI:2445438
• Synonyms: Rb^MI, Rb-Neo+
• Gene: Rb1 Location: Chr14:73430298-73563446 bp, - strand Genetic Position: Chr14, 38.73 cM, cytoband D3
• Alliance: Rb1^tm1Jyjw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80087
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Aspartic acid to alanine (D879A) and glycine to glutamic acid (G880E) mutations were introduced into the caspase cleavage site of exon 25 and a diagnostic EcoRI site was introduced into intron 24 via homologous recombination. A floxed neo cassette was also inserted into intron 25. Correctly targeted ES cells subjected to caspase activation did not show diminishment of Rb protein, thus confirming the mutation to the caspase cleavage site. (J:80087)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rb1 Mutation: 111 strains or lines available

References

• Original: J:80087 Chau BN, et al., Signal-dependent protection from apoptosis in mice expressing caspase-resistant Rb. Nat Cell Biol. 2002 Oct;4(10):757-65
• All: 1 reference(s)