Polk^tm1.1Rsky
Targeted Allele Detail

Summary

• Symbol: Polk^tm1.1Rsky
• Name: polymerase (DNA directed), kappa; targeted mutation 1.1, Klaus Rajewsky
• MGI ID: MGI:2445458
• Synonyms: Polk^tm1.1Esp
• Gene: Polk Location: Chr13:96617198-96679087 bp, - strand Genetic Position: Chr13, 50.56 cM, cytoband D1
• Alliance: Polk^tm1.1Rsky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80212
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP-flanked exon 6 and downstream floxed neo cassette were removed by crossing chimeric animals to a cre deleter strain resulting in the deletion of essential catalytic residues apartate 197 and glutamate 198, as well as a frameshift mutation via splicing from exon 5 to exon 7. RT-PCR analysis of testis RNA confirmed the presence of a shorter transcript in mutant animals. Northern blot analysis showed that the mutant transcript is expressed at 5 times less than the wild-type allele. (J:80212)

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Polk Mutation: 65 strains or lines available

References

• Original: J:80212 Schenten D, et al., DNA polymerase kappa deficiency does not affect somatic hypermutation in mice. Eur J Immunol. 2002 Nov;32(11):3152-60
• All: 5 reference(s)