Gja1^tm1Kwi
Targeted Allele Detail

Summary

• Symbol: Gja1^tm1Kwi
• Name: gap junction protein, alpha 1; targeted mutation 1, Klaus Willecke
• MGI ID: MGI:2445468
• Synonyms: 3lox, Cx43^fl, Cx43^fl[lacZ], Cx43::LacZ
• Gene: Gja1 Location: Chr10:56253297-56266519 bp, + strand Genetic Position: Chr10, 28.64 cM
• Alliance: Gja1^tm1Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78662
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites flanking the coding region of the gene were introduced into exon 2 and a floxed tk-neo cassette and lacZ gene were inserted into the downstream region via homologous recombination. Northern blot analysis confirmed expression of the mutant allele, and Western blot analysis showed protein expression is similar in mutant animals compared to wild-type. (J:78662)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gja1 Mutation: 60 strains or lines available

References

• Original: J:78662 Theis M, et al., Endothelium-specific replacement of the connexin43 coding region by a lacZ reporter gene. Genesis. 2001 Jan;29(1):1-13
• All: 114 reference(s)