Calca^tm1Hku
Targeted Allele Detail

Summary

• Symbol: Calca^tm1Hku
• Name: calcitonin/calcitonin-related polypeptide, alpha; targeted mutation 1, Hiroki Kurihara
• MGI ID: MGI:2445489
• Synonyms: alphaCGRP^-, CGRP^-
• Gene: Calca Location: Chr7:114230713-114235592 bp, - strand Genetic Position: Chr7, 59.99 cM
• Alliance: Calca^tm1Hku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80225
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The alphaCGRP sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT. (J:80225)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Calca^tm1Hku/Calca^tm1Hku	involves: 129S6/SvEvTac * C57BL/6

Phenotypes:

Affected Systems	hm1
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cardiovascular system	√
increased systemic vascular resistance	√
decreased heart rate variability	√
increased heart rate	√
increased mean systemic arterial blood pressure	√
homeostasis/metabolism	√
abnormal urine catecholamine level	√
nervous system	√
abnormal sympathetic nervous system physiology	√
renal/urinary system	√
abnormal urine catecholamine level	√
respiratory system	√
decreased airway responsiveness	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Calca Mutation: 23 strains or lines available

References

• Original: J:80225 Oh-hashi Y, et al., Elevated sympathetic nervous activity in mice deficient in alphaCGRP. Circ Res. 2001 Nov 23;89(11):983-90
• All: 25 reference(s)