Ptpra^tm1Cjp
Targeted Allele Detail

Summary

• Symbol: Ptpra^tm1Cjp
• Name: protein tyrosine phosphatase receptor type A; targeted mutation 1, Catherine J Pallen
• MGI ID: MGI:2445612
• Synonyms: PTPalpha-, PTPalpha^-
• Gene: Ptpra Location: Chr2:130292198-130396220 bp, + strand Genetic Position: Chr2, 63.23 cM, cytoband G
• Alliance: Ptpra^tm1Cjp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:55045
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A restriction fragment encompassing a 3' portion of exon 12 and all of exon 13 was replaced with a neomycin selection cassette inserted by homologous recombination. In addition to disrupting the downstream reading frame, the neo cassette replaces sequence around the junction of exon 13 and 14, which encodes the active site of the first catalytic domain. Protein containing an intact first catalytic domain was undetected by Western blot analysis of homozygous mutant brain lysates. (J:55045)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptpra Mutation: 45 strains or lines available

References

• Original: J:55045 Ponniah S, et al., Targeted disruption of the tyrosine phosphatase PTPalpha leads to constitutive downregulation of the kinases Src and Fyn. Curr Biol. 1999 May 20;9(10):535-8
• All: 9 reference(s)