Mybl2^tm1Sis
Targeted Allele Detail

Summary

• Symbol: Mybl2^tm1Sis
• Name: myeloblastosis oncogene-like 2; targeted mutation 1, Shunsuke Ishii
• MGI ID: MGI:2445746
• Synonyms: B-myb^-
• Gene: Mybl2 Location: Chr2:162896607-162926608 bp, + strand Genetic Position: Chr2, 84.0 cM
• Alliance: Mybl2^tm1Sis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57999
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5, which encodes amino acids 94 through 167, was replaced by the insertion of a neomycin selection cassette. The deleted region encompassed portions of repeats 2 and 3 that are involved in DNA binding. Whereas this allele was observed to be homozygously lethal, similar amounts of protein were detected by Western blot analysis of wild-type and heterozygous mice. (J:57999)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mybl2 Mutation: 114 strains or lines available

References

• Original: J:57999 Tanaka Y, et al., B-myb is required for inner cell mass formation at an early stage of development. J Biol Chem. 1999 Oct 1;274(40):28067-70
• All: 1 reference(s)