Etv4^tm1Hass
Targeted Allele Detail

Summary

• Symbol: Etv4^tm1Hass
• Name: ets variant 4; targeted mutation 1, John A Hassell
• MGI ID: MGI:2445834
• Synonyms: PEA3-, PEA3-null
• Gene: Etv4 Location: Chr11:101660568-101676197 bp, - strand Genetic Position: Chr11, 65.48 cM
• Alliance: Etv4^tm1Hass page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66012
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence extending from within exon 6 through exon 11, including the region encoding the ETS DNA binding domain, was replaced with a neomycin selection cassette inserted by homologous recombination. Though no normal or endogenous alternatively spliced transcripts were detected in homozygous mutant mice, a stable transcript corresponding to the targeted allele was identified by RNAse protection. Normal protein was undetected by Western blot analysis of MEFs, however, the lack of an amino-terminal antibody prevented the ruling out of the presence of a truncated protein. (J:66012)

Disease models

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Etv4 Mutation: 41 strains or lines available

References

• Original: J:66012 Laing MA, et al., Male sexual dysfunction in mice bearing targeted mutant alleles of the PEA3 ets gene. Mol Cell Biol. 2000 Dec;20(24):9337-45
• All: 9 reference(s)