Serpina5^tm1Gei
Targeted Allele Detail

Summary

• Symbol: Serpina5^tm1Gei
• Name: serine (or cysteine) peptidase inhibitor, clade A, member 5; targeted mutation 1, Margarethe Geiger
• MGI ID: MGI:2445835
• Synonyms: PCI^-
• Gene: Serpina5 Location: Chr12:104067372-104072396 bp, + strand Genetic Position: Chr12, 53.3 cM, cytoband F1
• Alliance: Serpina5^tm1Gei page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66422
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A 3.6 kb fragment containing a portion of exon 2, exons 3 and 4, and a portion of exon 5, was replaced by with a neomycin selection cassette. No transcript was detected in the testes or ovaries of homozygous mutant mice by RT-PCR analysis. Both Western blot analysis and ELISA indicated an absence of endogenous protein in the testes of homozygous mutant mice. (J:66422)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Serpina5 Mutation: 23 strains or lines available

References

• Original: J:66422 Uhrin P, et al., Disruption of the protein C inhibitor gene results in impaired spermatogenesis and male infertility. J Clin Invest. 2000 Dec;106(12):1531-9
• All: 2 reference(s)