Icos^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Icos^tm1Mak
• Name: inducible T cell co-stimulator; targeted mutation 1, Tak Mak
• MGI ID: MGI:2445839
• Synonyms: ICOS^-
• Gene: Icos Location: Chr1:61017086-61039479 bp, + strand Genetic Position: Chr1, 30.6 cM
• Alliance: Icos^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66702
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 2 and all of exons 3 and 4 were replaced by a neomycin selection cassette inserted by homologous recombination. The deleted region encoded the putative Icosl binding site, the transmembrane domain, and a portion of the intracellular domain including the YMFM motif that potentially binds lipid phosphotidylinositol 3 kinase. Flow cytometric analysis indicated an absence of protein on the surface of activated T cells obtained from homozygous mutant mice. (J:66702)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Icos Mutation: 37 strains or lines available

References

• Original: J:66702 Tafuri A, et al., ICOS is essential for effective T-helper-cell responses. Nature. 2001 Jan 4;409(6816):105-9
• All: 56 reference(s)