Arid5b^tm1Pjh
Targeted Allele Detail

Summary

• Symbol: Arid5b^tm1Pjh
• Name: AT-rich interaction domain 5B; targeted mutation 1, Paul J Hertzog
• MGI ID: MGI:2445941
• Synonyms: Desrt^-
• Gene: Arid5b Location: Chr10:67928350-68114570 bp, - strand Genetic Position: Chr10, 35.14 cM, cytoband B5.1
• Alliance: Arid5b^tm1Pjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70658
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 98 bp exon within the AT-rich interaction domain (ARID) was replaced with a neomycin selection cassette inserted by homologous recombination. RT-PCR of RNA obtained from primary embryonic fibroblasts confirmed the removal of the targeted exon. Sequence analysis identified a resultant frameshift mutation. (J:70658)

Expression

In Structures Affected by this Mutation:

16 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arid5b Mutation: 136 strains or lines available

References

• Original: J:70658 Lahoud MH, et al., Gene targeting of desrt, a novel arid class dna-binding protein, causes growth retardation and abnormal development of reproductive organs. Genome Res. 2001 Aug;11(8):1327-34
• All: 1 reference(s)