Tg(Prrx1-Hand2)5Eno
Transgene Detail

Summary

• Symbol: Tg(Prrx1-Hand2)5Eno
• Name: transgene insertion 5, Eric N Olson
• MGI ID: MGI:2446342
• Synonyms: RRR109-111EDE, Tg(Pr2x1-Hand2)5Eno
• Transgene: Tg(Prrx1-Hand2)5Eno Location: unknown
• Alliance: Tg(Prrx1-Hand2)5Eno page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prrx1-Hand2)5Eno expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hand2 (MGI:103580)

• Mutation details: The transgene consists of a mouse Prrx1 promoter fused to a mutant Hand2 cDNA with the following amino acid subsitutions: arginine to glutamic acid at residues 109 (R109E) and 111 (R111E), and arginine to aspartic acid at residue 110 (R110D). The mouse Prrx1 promoter was used to drive transgene expression in the forelimb bud and anterior mesoderm of the hindlimb in E10.5 embryos. The Hand2 construct also contains an N-terminal Myc epitope tag and a 3' human growth hormone polyadenylation signal. (J:80030)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:80030 McFadden DG, et al., Misexpression of dHAND induces ectopic digits in the developing limb bud in the absence of direct DNA binding. Development. 2002 Jul;129(13):3077-88
• All: 1 reference(s)