Tg(Prrx1-Hand2/VP16)1Eno
Transgene Detail

Summary

• Symbol: Tg(Prrx1-Hand2/VP16)1Eno
• Name: transgene insertion 1, Eric N Olson
• MGI ID: MGI:2446351
• Synonyms: prx-dHAND-VP16, Tg(Pr2x1-Hand2/VP16)1Eno
• Transgene: Tg(Prrx1-Hand2/VP16)1Eno Location: unknown
• Alliance: Tg(Prrx1-Hand2/VP16)1Eno page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prrx1-Hand2/VP16)1Eno expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hand2 (MGI:103580)

• Mutation details: The transgene consists of a mouse Prrx1 promoter and a Hand2 cDNA fused to the viral coactivator VP16 at the C-terminus. The mouse Prrx1 promoter was used to drive transgene expression in the forelimb bud and anterior mesoderm of the hindlimb in E10.5 embryos. The Hand2 construct also contains an N-terminal Myc epitope tag and a 3' human growth hormone polyadenylation signal. (J:80030)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:80030 McFadden DG, et al., Misexpression of dHAND induces ectopic digits in the developing limb bud in the absence of direct DNA binding. Development. 2002 Jul;129(13):3077-88
• All: 1 reference(s)