Nthl1^tm1Tbor
Targeted Allele Detail

Summary

• Symbol: Nthl1^tm1Tbor
• Name: nth (endonuclease III)-like 1 (E.coli); targeted mutation 1, George W Teebor
• MGI ID: MGI:2446391
• Synonyms: mNth1^-
• Gene: Nthl1 Location: Chr17:24851656-24857812 bp, + strand Genetic Position: Chr17, 12.42 cM, cytoband A3
• Alliance: Nthl1^tm1Tbor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78429
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encompassing exons 3 and 4 was replaced with a neomycin selection cassette inserted by homologous recombination. In addition to encoding the helix-hairpin-helix motif necessary for enzyme activty, the deleted region included codon 208, encoding a lysine orthologous to human lysine 212 which is a nucleophile that initiates the formation of a Schiff base ES intermediate. RT-PCR confirmed the absence of normal transcript in homozygous mutant mice. (J:78429)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nthl1 Mutation: 12 strains or lines available

References

• Original: J:78429 Ocampo MT, et al., Targeted deletion of mNth1 reveals a novel DNA repair enzyme activity. Mol Cell Biol. 2002 Sep;22(17):6111-21
• All: 2 reference(s)