Tg(CD2-Nfat5)1Snh
Transgene Detail

Summary

• Symbol: Tg(CD2-Nfat5)1Snh
• Name: transgene insertion 1, Steffan N Ho
• MGI ID: MGI:2446414
• Transgene: Tg(CD2-Nfat5)1Snh Location: unknown
• Alliance: Tg(CD2-Nfat5)1Snh page

Transgene origin

• Strain of Origin: (BALB/c x C57BL/6)F1

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD2-Nfat5)1Snh expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nfat5 (MGI:1859333)	Expresses a dominant negative form consisting of only the binding domain (amino acids 267-543)

• Tg(CD2-Nfat5)1Snh expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  CD2 (914)

  Cd2	(MGI:88320)

• Mutation details: A dominant negative form of mouse Nfat5 consisting of only the binding domain (amino acids 267-543) was cloned into a human CD2 expression vector and used as the transgene. The CD2 promoter is active in the thymus and mature peripheral T lymphocytes. Western blot analysis confirmed transgene expression in the thymus and spleen. (J:80066)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two high-expressing transgenic founders, CD2DBD2 and CD2DBD10, were created and used for analysis.

References

• Original: J:80066 Trama J, et al., The osmoprotective function of the NFAT5 transcription factor in T cell development and activation. J Immunol. 2002 Nov 15;169(10):5477-88
• All: 1 reference(s)