Tg(Ckmm-Ucp1)1Boud
Transgene Detail

Summary

• Symbol: Tg(Ckmm-Ucp1)1Boud
• Name: transgene insertion 1, Frederic Bouillaud
• MGI ID: MGI:2446436
• Synonyms: MCK-UCP1
• Transgene: Tg(Ckmm-Ucp1)1Boud Location: unknown
• Alliance: Tg(Ckmm-Ucp1)1Boud page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ckmm-Ucp1)1Boud expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Ucp1 (24860)

  Ucp1	(MGI:98894)

• Mutation details: The transgene consists of a rat Ucp1 cDNA sequence under the control of a mouse Ckmm promoter, which drove expression in skeletal muscle and heart. An SV40 polyadenylation signal was added to the Ucp1 construct. Transgene expression in heart and muscle tissues was confirmed by Northern blot analysis. (J:80053)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two transgenic lines, MCK-UCP1-13 and MCK-UCP1-20, were established. Both lines express the transgene in heart and muscle tissues, with expression levels varying in different muscles that is not consistent between the two lines.

References

• Original: J:80053 Couplan E, et al., High level of uncoupling protein 1 expression in muscle of transgenic mice selectively affects muscles at rest and decreases their IIb fiber content. J Biol Chem. 2002 Nov 8;277(45):43079-88
• All: 4 reference(s)